Part:BBa_K3711056
Panb1-α factor-crtE-AOX1 Terminator
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 124
Illegal XhoI site found at 649 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1325
- 1000COMPATIBLE WITH RFC[1000]
Description
This is a composite part for extracellular expression of Geranylgeranyl pyrophosphate synthase. crtE is expressed and participates in the production from Farnessee pyrophosphate (FPP) to Geranylgeranyl pyrophosphate (GGPP). Panb1 is a constitutive promoter in yeast, which is expressed under anaerobic conditions, while under aerobic conditions, Panb1, as a repression target of ROX1, is inhibited. When Panb1 initiates the expression, the signal peptide, α-factor, is used to express crtE outside of the cell.
Usage and Biology
crtE is derived from Erwinia, encodes Geranylgeranyl pyrophosphate synthase and participates in the synthesis of carotenoids. The early steps of carotenoid biosynthesis pathway include the synthesis of Geranylgeranyl pyrophosphate (GGPP), the condensation of two molecules of GGPP into octahydrolycopene and desaturation of octahydrolycopene into plant fluorene, β-carotene, protolycopene and lycopene. The crtE encodes Geranylgeranyl pyrophosphate synthase which synthesizes GGPP. Laboratory studies have shown that E.coli transformed with E.herbicola carotenoid synthesis gene could resist higher level of LTV radiation and phototoxic environment, indicating that the synthesis of carotenoid may be very important for the survival of E.herbicola in nature.
Molecular cloning
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